1.無(wú)菌室內(nèi)的工作台,不論是什麽材質、用途的(de),都(dōu)要求表麵光滑和台麵水平。
1. The worktable in aseptic room, no matter what material and use, requires smooth surface and horizontal table.
2.在內室和外室各安裝一個紫外燈(多為30W)。內室(shì)的紫外線燈應安裝在經常工(gōng)作的座位正上方,離地麵2m,外室的(de)紫外線燈可安裝在外室。
2. Install an ultraviolet lamp (mostly 30W) in the inner and outer chambers. The ultraviolet lamp in the inner room should be installed directly above the working seat, 2m away from the ground, and the ultraviolet lamp in the outer room can be installed
in the center of the outer room.
3.外室應有(yǒu)專用的工作服、鞋、帽、口罩、盛有來蘇兒水的瓷盆和毛巾、手持噴霧器和5%石炭酸溶液等(děng)。
3. the outer rooms should have special work clothes, shoes, caps, masks, pottery basins and towels containing the suer water, hand held sprayers and 5% carbolic acid solutions.
4.內室應有酒精燈、常用接種工具、不鏽鋼製的刀、剪(jiǎn)、鑷子、70%的(de)酒精棉球、工業酒精、載玻璃片、特種(zhǒng)蠟筆、記錄本(běn)、鉛筆、標簽紙、膠水、廢物筐(kuāng)等。
4. There should be alcohol lamp, common inoculation tools, stainless steel knife, scissors, tweezers, 70% alcohol cotton ball, industrial alcohol, glass carrier, special crayon, record book, pencil, label paper, glue, waste basket, etc.
無菌室(shì)的滅菌消毒
Sterilization of aseptic room
1.薰蒸:這(zhè)是無菌室徹底(dǐ)滅(miè)菌的措施。無菌室使用了較長時間,汙(wū)染(rǎn)比較嚴重時,應(yīng)進行薰蒸滅菌。可用甲(jiǎ)醛、乳酸或硫磺薰蒸。
1. Fumigation: This is a measure to completely sterilize the sterile room. If the aseptic room has been used for a long time and the pollution is serious, it should be fumigated and sterilized. It can be fumigated with formaldehyde, lactic acid or sulfur.
2.噴霧:在(zài)每(měi)次使用無菌室前(qián)進行。噴霧可促使空氣中微粒(lì)及微生物沉降,防止桌麵、地麵上的微塵飛場,並有殺菌作用。可用5%石炭酸噴霧。
2. spray: before the use of asepsis room. Spray can cause particles and microorganisms to settle in the air, preventing dust flying on the desktop and ground, and having bactericidal effect. It can be sprayed with 5% carbolic acid.
3.紫外線照(zhào)射:在每次(cì)使用無菌室前進行(háng)。紫外線有較好的殺菌效果。通常(cháng)應開啟紫外線燈照射30~60min。
3. Ultraviolet radiation: before each use of sterile room. UV has good germicidal efficacy. Generally, the ultraviolet lamp should be turned on to irradiate for 30-60min.
無菌室工作規程
Working procedure of aseptic room
1.無菌室滅菌。每(měi)次使用前開啟紫外線燈照射30min以上(shàng),或在使用前30min,對內外室(shì)用5%石炭酸噴霧。
1. Sterilization in aseptic room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
2.用肥皂洗手後,把所需器材(cái)搬入外室(shì);在(zài)外室換上已(yǐ)滅菌的工作服、工作帽和工作(zuò)鞋,戴好口罩,然後用2%煤酚皂液將手浸洗2分(fèn)鍾。
2. After washing hands with soap, move the required equipment into the outer room; change into sterilized work clothes, work caps and work shoes in the outer room, wear masks, and then soak hands with 2% coal phenol soap for 2 minutes.
3.將各種(zhǒng)需用物品搬進內室清點、就位,用(yòng)5%石炭酸在工作台(tái)麵(miàn)上方和操作員站位空間噴霧,返回外室,5~10min後再進內室工作。
3. move all necessary articles into the inner room for inventory and position. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
4.接種操作前,用70%酒(jiǔ)精棉球擦手;進行無菌操(cāo)作時,動作要輕(qīng)緩,盡量減少空氣波動和(hé)地麵揚塵。
4. Before inoculation, wipe hands with 70% alcohol cotton ball; during aseptic operation, the action should be gentle to minimize air fluctuation and ground dust.
5.工作中應注意安全。如遇棉塞著火,用(yòng)手(shǒu)緊握或用濕布包裹熄滅,切勿用嘴吹,以免擴大燃燒;如遇有菌培養物灑落或打碎(suì)有菌容器時,應(yīng)用浸潤5%石炭(tàn)酸的抹布包裹後,並用浸潤5%石炭酸的(de)抹布擦拭台麵或地麵,用酒精(jīng)棉球擦手後再繼續操作。
5. Pay attention to safety in work. If the cotton plug is on fire, hold it tightly with your hands or wrap it with a wet cloth. Do not blow it with your mouth to avoid expanding combustion. In case of bacterial culture spilled or broken, wrap it with
a cloth soaked in 5% carbolic acid, and wipe the table or ground with a cloth soaked in 5% carbolic acid, and then continue to operate after wiping hands with alcohol cotton ball.
6.工(gōng)作結(jié)束,立即將台麵(miàn)收拾幹淨,將不應在(zài)無(wú)菌室存放(fàng)的物品和廢棄物全部拿出(chū)無菌室後,對無菌室用5%石炭酸噴(pēn)霧,或開紫外線燈照射(shè)30min。
6. at the end of the work, clean up the table immediately, and remove all the articles and wastes that should not be stored in the asepsis room, then use the 5% carbolic acid spray or the ultraviolet lamp to irradiate 30min.
昆山昌(chāng)瑞空調淨化技(jì)術有限公司,專注(zhù)從事空氣過濾器(qì)係列產(chǎn)品(初/中/高效、化學過濾(lǜ)器等)、潔淨(jìng)室淨(jìng)化設備(拋棄式/可(kě)更換式/自動卷繞式(shì)過濾器、高效送風口、FFU高效(xiào)過濾器淨(jìng)化機組)的研發、生產、銷售和售後(hòu)。
Changrui Air Conditioning Purification Technology Co., Ltd. focuses on the R&D, production, sales and after-sales of air filter series products (primary/medium/high-efficiency, chemical filters, etc.) and clean room purification equipment (disposable/replaceable/automatic
winding filters, high-efficiency air supply outlets, FFU high-efficiency filter purification units).